Quantitative determination of ergot alkaloids in biological fluids by radioimmunoassay.
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چکیده
منابع مشابه
Identification and determination of ergot alkaloids in Morning Glory cultivars.
Seeds of plants from Ipomoea genera contain numerous ergot alkaloids, including psychoactive ergine and ergometrine, and are often abused as so-called "legal highs." In this work, an analytical method for determination of ergine and ergometrine, and identification of other alkaloids was developed, optimized, and validated. Three extraction techniques, ultrasound-assisted extraction in bath, or ...
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متن کاملAnalysis of Ergot Alkaloids
The principles and application of established and newer methods for the quantitative and semi-quantitative determination of ergot alkaloids in food, feed, plant materials and animal tissues are reviewed. The techniques of sampling, extraction, clean-up, detection, quantification and validation are described. The major procedures for ergot alkaloid analysis comprise liquid chromatography with ta...
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immunofixation (2), and beta-mercaptoethanol reduction (3) of serum, with subsequent zone electrophoresis (Figure 1). In each instance, such reduction caused the band to disappear. The immunofixation and immunoelectrophresis procedures confirmed the presence of polymeric, polyclonal 1gM. One would expect that a discrete band would still be present on zone electrophoresis after reduction if 1gM ...
متن کاملBiosynthetic Pathways of Ergot Alkaloids
Ergot alkaloids are nitrogen-containing natural products belonging to indole alkaloids. The best known producers are fungi of the phylum Ascomycota, e.g., Claviceps, Epichloë, Penicillium and Aspergillus species. According to their structures, ergot alkaloids can be divided into three groups: clavines, lysergic acid amides and peptides (ergopeptines). All of them share the first biosynthetic st...
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ژورنال
عنوان ژورنال: British Journal of Clinical Pharmacology
سال: 1978
ISSN: 0306-5251
DOI: 10.1111/j.1365-2125.1978.tb04594.x